collected and transported in the ESwab, with, urethral swabs and 10 wound swabs). Because samples are frequently submitted on swabs from distant sites, viability of the organism must be maintained. The methanol. The slides were prepared, stained and observed as, Comparison of proportion was performed with a two-, sided Fisher's exact test. Composition of Amies Transport Medium Final pH (at 25°C) 7.2±0.2 Principle of Amies Transport Medium Amies Transport Medium provides a reduced environment due to the presence of sodium thioglycollate and the small amount of agar. 3,4,5. There were no significant differences in the recovery rates of the two systems. Because in the Nugent system several cell types are not taken into account for a final score, we carried out a detailed assessment of the composition of the vaginal microflora in relation to standard Gram stain in order the improve the diagnostic value of the Gram stain. (adsbygoogle = window.adsbygoogle || []).push({}); Ames transport is prepared from dehydrated medium obtained from manufacturer with instructions. 2015 2.484 Change of Procedure for Transport of GC/Chlamydia Probe Testing, Evaluation of two transport systems for gonorrhea cultures. Microscopic examination of 240, d 160 with ESwab) showed that the quality of smear preparation, valent to those prepared when received in the, l, shows superior results to those obtained using the Amies gel. (15 isolates with ESwab vs. 7 isolates with Amies gel swab, p = 0.005) with ESwab (quantitative method). KVM International A/S-Kjellerup/Denmark exhibited splitting machines with working widths of up to 1,200 m. The machine includes an easy snap-blade change in combination with the respective transport system. 0000015185 00000 n In this paper, we illustrate how LBM can positively change laboratory workflow by illustrating several years of our experience with LBM. 0000011359 00000 n 0000010291 00000 n Microscopic examination performed using ESwab, especially when preparing the slides with 100 mul, shows superior results to those obtained using the Amies gel Transystem. Besser Company-Alpena/USA& Besser/Omag-Emden/Germany presented a newly developed Rustic Surface Aging Machine, which provides paving blocks a distinctive, weathered look. Skin presence was found in 30% of animals with the highest isolation rates found at the carpus (16.7%), neck, withers and croup (13.3% each). LB: Lactobacilli; BG-: Gram-negative bacilli; DC+: Gram-. Both the swabs and the urine samples may be transported to the laboratory at room temperature. In addition, LBM offers advantages in the efficiency of microorganism recovery and ease of sampling, transport, and storage. While Q score, applied for wounds, moved, towards a positive value due to the observation of poly-, morphonuclear cells (lacking in Amies gel slides). All authors approved the final version of the manuscript. An ideal swab system must have. A total of 515 samples were obtained from 197 pregnant women, of which 403 (78.3%) were categorized as grade I microflora, 46 (8.9%) as grade II, 22 (4.3%) as grade III and 8 (1.6%) as grade IV, according to the criteria of Ison and Hay. nce-Landmark Impact Factor The results demonstrate the efficiency of the proposed method. Triplicate swabs were seeded with 3 dilutions of Neisseria gonorrhoeae, Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae. <<4C89159BFE19574DB45558E6C2C9D937>]/Prev 121934/XRefStm 1465>> 0000021900 00000 n The first step in routine microbiology laboratory procedures is the collection and safe transportation of swab samples. A unique collection device for several investigations also guarantees quality due to the uniformity of the sample and standardization of procedures. Access scientific knowledge from anywhere. 0000000016 00000 n Screw the cap on firmly. The Gram stain can be a, critical test for the rapid presumptive diagnosis of infec-, tious agents and serve to assessment of the quality of clin-, ical specimens [1-6]. 0000022786 00000 n 2016 2.497 (Becton Dickinson, Becton Drive Franklin Lakes, NJ, USA). Transport swabs and microbiology swabs: A mix of throat swabs, pertusis swabs, culture swabs, charcoal swabs, nasopharyngeal swabs with amies transport medium. All slides were gram stained using an automated Gram stainer. % of Medium (per litre) Classification MOPS Acid 1132-61-2 0.315 % R36/37/38 LBM allows clinical specimen optimization and has several important advantages: cost reduction (due to the smaller number of different devices used), time savings for medical or nursing staff (less confusion in collection device selection and fewer samples being collected), time savings for laboratory staff (fewer samples to access and handle for individual investigations), and patient comfort improvement (multiple sample collection can be avoided). With N. meningitidis and Haemophilus, higher levels of recovery were clearly obtained with Copan (P < 0.05 to P < 0.001). FC contributed to the review of the study and data, analysis. Guest Editors: Dr. Manuel Scimeca and Prof. Elena Bonanno This is a physical characteristic caused by its chemical composition and is normal. The results of sample analysis concerning blood gases, electrolytes and haemoglobin were compared and examined for differences. 0000025852 00000 n In this paper, the Gram-staining technique is used as a tool for the differentiation of Gram-positive and Gram-negative bacteria, as a first step to determine the identity of a particular bacterial sample. Specimens were transported directly to the lab-, oratory (within 2 h). The skin is colonized by a diverse collection of microorganisms which, for the most part, peacefully coexist with their hosts. We confirmed that ESwab has a high performance for Gram stains and a higher bacterial recovery than dry and Amies gel swabs when using clinical samples for MRSA screening and wound sampling. We thank Copan Italia for providing ESwab system for the evaluation. Once a swab sample was collected, it was placed immedi-, ately into the ESwab transport tube containing transport, medium. 0000019106 00000 n (44 isolates with ESwab vs. 29 with Amies gel swab, p = 0.001) and Enterococcus spp. startxref was drained off, and the slides were air-dried. 0000010701 00000 n h�bb�f`b``Ń3� ����0 �@/ 305 0 obj <>stream evaluate the ESwab system with regards to the Gram-stain. De Ganck C, De Backer E, Temmerman M, Vaneechoutte M: parison between Gram stain and culture for the characteri-. To ensure, accuracy of interpretation, Gram stains were viewed by, two different competent persons and reviewed by a super-, visor. 265 41 The remaining portion of the. Two swabs were simultaneously collected from, each patient, the first one using the conventional rayon, swab of the Amies gel Transystem, the other using the. © 2009 Fontana et al; licensee BioMed Central Ltd. Our findings showed, that the slides prepared from ESwab system were observed, to demonstrate more human cell and bacterial species, than the traditional Amies gel system. In particular , the Eswab system (by Copan) simplifies and streamlines specimen collection and represents the only liquid system supporting the recovery of all types of bacteria (aerobic, anaerobic, and fastidious bacteria). Bacterial recovery was significantly higher with ESwab (p < 0.01, for both MRSA screening and wounds, quantitative/semi-quantitative method). Mix by inversion 3-4 times. trailer aerobic, anaerobic, and fastidious bacteria such as. 265 0 obj <> endobj Journal Abbreviation: FRONT BIOSCI-LANDMRK collection supplies are ordered.