Rabbit Anti-Wheat Germ Agglutinin antibody (ab178444) Wheat Germ Agglutinin/Phalloidin Cocktail - Add 10 microliters of WGA stock solution (1 milligram per milliliter) and 20 microliters of phalloidin stock solution (6.6 micromolar) to 1 milliliter of BSA Blocking Buffer. This method of staining and analysis of cardiomyocytes enables accurate morphometric measurements in thick histological sections, thus unlocking the potential of archived tissue. Get resources and offers direct to your inbox. Discussion.

WGA is a N acetylglucosamine specific lectin and is synthesized in developing and germinating embryos, roots and meristematic part of the leaves. Wheat germ agglutinin (WGA) is a lectin that protects wheat from insects, yeast and bacteria.An agglutinin protein, it binds to N-acetyl-D-glucosamine and Sialic acid. Your browser does not have JavaScript enabled and some parts of this website will not work without it. Wash the counterstained specimens with either PBSA or Hanks Balanced Salt Solution (depending upon the nuclear dye) for three times at 5 minutes for each wash.

For thin sections (5-10 micrometers) block for one hour, and for thick sections (10-20 micrometers) block for two hours. WGA has been reported to have a role in dormancy and plant defence, but its … Slowly rotate the tissue sections as they are being permeabilized on an orbital shaker at 5-10 revolutions per minute. Lectin interaction with certain carbohydrates are as specific as enzyme to substrate or antigen to antibody interactions and have been used to study protein glycosylation. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. WGA has been reported to have a role in dormancy and plant defence, but its characteristic biochemical properties and its predominance in growing and meristematic tissues, and its osmotic- and drought-stress inducibility suggest that this protein might be involved in a much wider range of functions. FWGA was first used as an alternative to the Gram stain and used to distinguish Gram-negative and Gram-positive bacteria (Sizemore, Caldwell, & Kendrick, 1990). If you entered the URL manually, please check your spelling and try again. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. After blocking, remove the slides from the staining jar and carefully wipe the edges and back of the glass to remove excess Blocking Buffer. The histogram analysis of WGA-FITC stained heart A (Figure 5C) shows a large overlap between background histogram (black border) and complete heart histogram (red border) in the range between brightness level 25 and 70. Nuclei were counterstained with Hoechst 33342. Scale bars, 100 or 50 μm as indicated. After fixation, wash the tissue sections with three changes of PBSA Wash Buffer. (C) Histological examination of cardiac hypertrophy by TRITC-WGA staining (upper). (C) combined image of CD31-Cy3 (red) and WGA-fluorescein (green) staining. Our Abpromise guarantee covers the use of Avoid freeze / thaw cycle. Each thin section receives 250-500 microliters of staining cocktail. Publishing research using ab178444?

Nuclear Dye - Prepare fresh dilutions of the nuclear dye immediately prior to staining. Block nonspecific fluorophore binding sites with Blocking Buffer. WGA is a protein found abundantly in wheat kernels. Cover the humidity chamber with aluminum foil to protect the fluorophores from light. Dissolve the membranes in the tissue sections with Permeabilization Buffer by treatment for one or two hours for thin (5-10 micrometer) and thick (10-20 micrometer) sections, respectively. Counterstaining for nuclei using a variety of popular DNA-binding dyes follows treatment with the lectin and actin probes. There are currently no Customer reviews or Questions for ab178444.Please use the links above to contact us or submit feedback about this product. Cover the staining jar with aluminum foil (to protect from light) and slowly rotate the tissue sections as they are being washed on an orbital shaker at 5-10 revolutions per minute. ab178444 Fluorescent WGA lectin co-staining of bone, ligament and tendon sections (A) a section of a palm from 3-day old rat was stained with antibodies against CD31-Cy3 (red) to visualize blood vessels. 4 Department of ... Confocal immunofluorescence after WGA (green), DAPI (blue) stains, and BrdU antibodies revealed proliferating non-myocyte cells (magenta nuclei, arrowheads) in regions with increased fibrosis (bottom panel, original magnification, ×400). Apply a sufficient amount of labeling solution to cover cells adhering to coverslip(s). Please contact us to place your order, or try again later. Scale bar, 0.5 cm. the optimal conditions for tissue processing, staining, and microscope observations. When labeling is complete, remove the labeling solution, and wash the cells twice 7A. Question. Slowly rotate the tissue sections as they are being washed on an orbital shaker at 5-10 revolutions per minute. I am trying to stain mice whole heart sections with WGA for cardiac myocyte size estimation and IB4 for microvascular density. 3 Heart Institute, Hadassah Hebrew University Medical Center, Jerusalem, Israel. We use cookies to make our site as useful as possible.

ab178444 has not yet been referenced specifically in any publications. … Protect from light with aluminum foil. Goat Anti-Rabbit IgG H&L (HRP) (ab205718) The page you requested cannot be found. Both of these techniques use antibodies to detect specific … (A,B) Identification of capillaries by WGA-AF488 enhancing staining. Incubate the covered slides in the humidity chamber for one or two hours (thin and thick specimens, respectively) at 37 degrees. 4 answers. View complete list of fluorescent dye-conjugated lectins › in the following tested applications. The ratios of HW/BW and HW/TL (lower) (n = 6 per group). NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact [email protected], Rabbit polyclonal to Wheat Germ Agglutinin. Slowly rotate the tissue sections as they are being fixed on an orbital shaker at 5-10 revolutions per minute. IHC-P, WB, ELISA, IP. These two words are now being used interchangeably in scientific publications and presentations. On the right (Panel B) DAPI appears in blue, WGA-AF488 appears in green. Alexa Fluor® 488 WGA binds to sialic acid and N-acetylglucosaminyl residues. Triticum vulgare Wheat germ agglutinin. Presented in Figure 1 is a confocal image revealing striated actin fiber bundle structure in a thick (16 micrometer) section of rat tongue. 2.5 Wash cells. 2.4 Label cells. FWGA can easily … The specimen was imaged with a 60x oil immersion objective (without zoom) using a 405-nanometer violet diode laser (Hoechst), a 488-nanometer argon-ion laser (Oregon Green 488), and a 543-nanometer helium-neon laser. DAPI (blue) … Blocking Buffer - 1 percent bovine serum albumen (BSA) in PBSA containing 0.05 percent Triton X-100 (add 2-3 milligrams sodium azide per 100 milliliters of blocking buffer to eliminate the growth of microorganisms). Fixative - 3.7 percent paraformaldehyde in PBSA (prepare fresh fixer each day). The section was stained as detailed below using wheat germ agglutinin conjugated to Alexa Fluor 568 and phalloidin conjugated to Oregon Green 488. DAPI - 5 microliters in 150 milliliters of PBSA (5 minutes). On the left (Panel A) DAPI appears in yellow/red, WGA-AF488 appears in green. Among the useful fluorescent markers for visualization of tissues are rhodamine, fluorescein, the Alexa Fluor series, and the cyanine dyes. SYTOX Green and Orange - 10 microliters in 250 milliliters of Hanks BSS (30 minutes). © 1998-2020 Abcam plc. (B) the same section shown in A was co-stained with WGA-fluorescein (green) to outline general tissue structure. Upon delivery aliquot. This protocol details a generalized procedure for staining tissue cryosections ranging from 5 to 20 micrometers in thickness. WGA is a N acetylglucosamine specific lectin and is synthesized in developing and germinating embryos, roots and meristematic part of the leaves. For DAPI and cyanine nuclear counterstains, add the diluted dye in PBSA to the staining jar and treat the specimen for the recommended time: 5-10 minutes for DAPI; 15-30 minutes for cyanine dyes (protect from light with aluminum foil). The following resources may help you locate the website you're looking for: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z, 1000 E. University Ave. Laramie, WY 82071UW Operators (307) 766-1121 | Contact Us | Download Adobe Reader, Accreditation | Emergency Preparedness | Employment at UW | Gainfull Employment | Privacy Policy | Accessibility. Figure 8 . We recommended a WGA conjuage concentration of 5.0 µg/mL.